The video shows the early development of a zebrafish embryo imaged by a light sheet microscope. The time in the left upper corner shows the hours and minutes post fertilization.
LSFM (Light Sheet Fluorescent Microscopy), also known as SPIM (Selective Plane Illumination Microscopy), is a method of imaging that uses a light sheet to illuminate a sample from the side in the focal plane of the detection objective (Fig. 1).
For more information, visit Huisken Lab website.
Rory M Power, and Jan Huisken. “A Guide to Light-Sheet Fluorescence Microscopy for Multiscale Imaging.” Nature Methods, vol. 14, no. 4, 2017, p. 360.
Emmanuel G Reynaud, et al. “Guide to Light-Sheet Microscopy for Adventurous Biologists.” Nature Methods, vol. 12, no. 1, 2014, p. 30.