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LSFM (Light Sheet Fluorescent Microscopy)

This is a list of publications on the theory, design and applications of light sheet microscopy and similar techniques. We try to keep it up-to-date, but please email Susi ( if a paper is missing.

UW-Madison LSFM Contact

Susi Power

Science Communication Specialist for the Huisken group (UW-Madison)

"Feel free to contact me regarding your LSFM inquires or requests."

Long-term imaging of Zebrafish with light sheet microscopy

The video shows the early development of a zebrafish embryo imaged by a light sheet microscope. The time in the left upper corner shows the hours and minutes post fertilization. 

What is LSFM?

LSFM (Light Sheet Fluorescent Microscopy), also known as SPIM (Selective Plane Illumination Microscopy), is a method of imaging that uses a light sheet to illuminate a sample from the side in the focal plane of the detection objective (Fig. 1). 

For more information, visit Huisken Lab website.

Get Started

A Guide to Light-Sheet Fluorescence Microscopy for Multiscale Imaging.

Rory M Power, and Jan Huisken. “A Guide to Light-Sheet Fluorescence Microscopy for Multiscale Imaging.” Nature Methods, vol. 14, no. 4, 2017, p. 360.

Guide to Light-Sheet Microscopy for Adventurous Biologists

Emmanuel G Reynaud, et al. “Guide to Light-Sheet Microscopy for Adventurous Biologists.” Nature Methods, vol. 12, no. 1, 2014, p. 30.