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LSFM (Light Sheet Fluorescent Microscopy)

This is a list of publications on the theory, design and applications of light sheet microscopy and similar techniques. We try to keep it up-to-date, but please email Susi (spower@morgridge.org) if a paper is missing.

2011 SPIM Publications

Capoulade, J., Wachsmuth, M., Hufnagel, L., & Knop, M. (2011). Quantitative fluorescence imaging of protein diffusion and interaction in living cells. Nat. Biotechnol., 29(9), 835–839. https://doi.org/10.1038/nbt.1928


Cella Zanacchi, F., Lavagnino, Z., Perrone Donnorso, M., Del Bue, A., Furia, L., Faretta, M., & Diaspro, A. (2011). Live-cell 3D super-resolution imaging in thick biological samples. Nat. Methods, 8(12), 1047–1049. https://doi.org/10.1038/nmeth.1744


Cella Zanacchi, F., Lavagnino, Z., Pesce, M., Difato, F., Ronzitti, E., & Diaspro, A. (2011). Two-photon fluorescence excitation within a light sheet based microscopy architecture. In SPIE BiOS (p. 79032W–79032W–5). International Society for Optics and Photonics. https://doi.org/10.1117/12.879792


Friedrich, M., Gan, Q., Ermolayev, V., & Harms, G. S. (2011). STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution. Biophys. J., 100(8), L43–5. https://doi.org/10.1016/j.bpj.2010.12.3748


Friedrich, M., Nozadze, R., de Keijzer, S., Steinmeyer, R., Ermolayev, V., & Harms, G. S. (2011). Detection of Single Quantum Dots in Model Systems with Sheet Illumination Microscopy. J. Fluoresc. https://doi.org/10.1007/s10895-011-0966-4


Greger, K., Neetz, M. J., Reynaud, E. G., & Stelzer, E. H. K. (2011). Three-dimensional Fluorescence Lifetime Imaging with a Single Plane Illumination Microscope provides an improved signal to noise ratio. Opt. Express, 19(21), 20743–20750. https://doi.org/10.1364/OE.19.020743


Huotari, S., Pylkkänen, T., Verbeni, R., Monaco, G., & Hämäläinen, K. (2011). Direct tomography with chemical-bond contrast. Nat. Mater., 10(7), 489–493. https://doi.org/10.1038/nmat3031


Keller, P. J., Schmidt, A. D., Wittbrodt, J., & Stelzer, E. H. K. (2011). Digital scanned laser light-sheet fluorescence microscopy (DSLM) of zebrafish and Drosophila embryonic development. Cold Spring Harb. Protoc., 2011(10), 1235–1243. https://doi.org/10.1101/pdb.prot065839


Khairy, K., & Keller, P. J. (2011). Reconstructing embryonic development. Genesis, 49(7), 488–513. https://doi.org/10.1002/dvg.20698


Kumar, S., Wilding, D., Sikkel, M. B., Lyon, A. R., MacLeod, K. T., & Dunsby, C. (2011a). Application of oblique plane microscopy to high speed live cell imaging. In European Conference on Biomedical Optics (p. 80860V). Optical Society of America. https://doi.org/10.1364/ECBO.2011.80860V


Kumar, S., Wilding, D., Sikkel, M. B., Lyon, A. R., MacLeod, K. T., & Dunsby, C. (2011b). High-speed 2D and 3D fluorescence microscopy of cardiac myocytes. Opt. Express, 19(15), 13839–13847. https://doi.org/10.1364/OE.19.013839


Lorenzo, C., Frongia, C., Jorand, R., Fehrenbach, J., Weiss, P., Maandhui, A., … Lobjois, V. (2011). Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy. Cell Div., 6, 22. https://doi.org/10.1186/1747-1028-6-22


Maizel, A., von Wangenheim, D., Federici, F., Haseloff, J., & Stelzer, E. H. K. (2011). High-resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy. Plant J., 68(2), 377–385. https://doi.org/10.1111/j.1365-313X.2011.04692.x


Mertz, J. (2011). Optical sectioning microscopy with planar or structured illumination. Nat. Methods, 8(10), 811–819. https://doi.org/10.1038/nmeth.1709


Planchon, T. A., Gao, L., Milkie, D. E., Davidson, M. W., Galbraith, J. A., Galbraith, C. G., & Betzig, E. (2011). Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination. Nat. Methods, 8(5), 417–423. https://doi.org/10.1038/nmeth.1586


Santi, P. A. (2011). Light sheet fluorescence microscopy: a review. J. Histochem. Cytochem., 59(2), 129–138. https://doi.org/10.1369/0022155410394857


Sena, G., Frentz, Z., Birnbaum, K. D., & Leibler, S. (2011). Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy. PLoS One, 6(6), e21303. https://doi.org/10.1371/journal.pone.0021303


Swoger, J., Muzzopappa, M., López-Schier, H., & Sharpe, J. (2011). 4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies. J. Biophotonics, 4(1–2), 122–134. https://doi.org/10.1002/jbio.201000087


Taylor, J. M., Saunter, C. D., Love, G. D., Girkin, J. M., Henderson, D. J., & Chaudhry, B. (2011). Real-time optical gating for three-dimensional beating heart imaging. J. Biomed. Opt., 16(11), 116021. https://doi.org/10.1117/1.3652892


Temerinac-Ott, M., Ronneberger, O., Nitschke, R., Driever, W., & Burkhardt, H. (2011). Spatially-variant Lucy-Richardson deconvolution for multiview fusion of microscopical 3D images. In Biomedical Imaging: From Nano to Macro, 2011 IEEE International Symposium on (pp. 899–904). https://doi.org/10.1109/ISBI.2011.5872549


Tomer, R., Khairy, K., & Keller, P. J. (2011). Shedding light on the system: studying embryonic development with light sheet microscopy. Curr. Opin. Genet. Dev., 21(5), 558–565. https://doi.org/10.1016/j.gde.2011.07.003


Truong, T. V., Supatto, W., Koos, D. S., Choi, J. M., & Fraser, S. E. (2011). Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nat. Methods, 8(9), 757–760. https://doi.org/10.1038/nmeth.1652


Weber, M., & Huisken, J. (2011). Light sheet microscopy for real-time developmental biology. Curr. Opin. Genet. Dev., 21(5), 566–572. https://doi.org/10.1016/j.gde.2011.09.009


Wu, Y., Ghitani, A., Christensen, R., Santella, A., Du, Z., Rondeau, G., … Shroff, H. (2011). Inverted selective plane illumination microscopy (iSPIM) enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U. S. A., 108(43), 17708–17713. https://doi.org/10.1073/pnas.1108494108


Xu, Z., & Holy, T. E. (2011). Development of low-coherence light sheet illumination microscope for fluorescence-free bioimaging. In SPIE Optical Engineering + Applications (pp. 812908–812908–10). International Society for Optics and Photonics. https://doi.org/10.1117/12.893569