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LSFM (Light Sheet Fluorescent Microscopy)

This is a list of publications on the theory, design and applications of light sheet microscopy and similar techniques. We try to keep it up-to-date, but please email Susi (spower@morgridge.org) if a paper is missing.

2008 SPIM Publications

Becker, K., Jährling, N., Kramer, E. R., Schnorrer, F., & Dodt, H.-U. (2008). Ultramicroscopy: 3D reconstruction of large microscopical specimens. J. Biophotonics, 1(1), 36–42. https://doi.org/10.1002/jbio.200710011


Cavey, M., & Lecuit, T. (2008). Imaging cellular and molecular dynamics in live embryos using fluorescent proteins. Methods Mol. Biol., 420, 219–238. https://doi.org/10.1007/978-1-59745-583-1_13


Chi, N. C., Shaw, R. M., Jungblut, B., Huisken, J., Ferrer, T., Arnaout, R., … Stainier, D. Y. R. (2008). Genetic and physiologic dissection of the vertebrate cardiac conduction system. PLoS Biol., 6(5), e109. https://doi.org/10.1371/journal.pbio.0060109


Dunsby, C. (2008). Optically sectioned imaging by oblique plane microscopy. Opt. Express, 16(25), 20306–20316.


Engelbrecht, C. J., Johnston, R. S., Seibel, E. J., & Helmchen, F. (2008). Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo. Opt. Express, 16(8), 5556–5564.


Greger, K. (2008). Dynamic 3D Fluorescence Lifetime Imaging using Selective Plane Illumination Microscopy (SPIM). In FOM 2008 (pp. 1–1).


Hofman, R., Segenhout, J. M., Buytaert, J. A. N., Dirckx, J. J. J., & Wit, H. P. (2008). Morphology and function of Bast’s valve: additional insight in its functioning using 3D-reconstruction. Eur. Arch. Otorhinolaryngol., 265(2), 153–157. https://doi.org/10.1007/s00405-007-0424-8


Holekamp, T. F., Turaga, D., & Holy, T. E. (2008). Fast three-dimensional fluorescence imaging of activity in neural populations by objective-coupled planar illumination microscopy. Neuron, 57(5), 661–672. https://doi.org/10.1016/j.neuron.2008.01.011


Keller, P. J., Pampaloni, F., Lattanzi, G., & Stelzer, E. H. K. (2008). Three-dimensional microtubule behavior in Xenopus egg extracts reveals four dynamic states and state-dependent elastic properties. Biophys. J., 95(3), 1474–1486. https://doi.org/10.1529/biophysj.107.128223


Keller, P. J., Schmidt, A. D., Wittbrodt, J., & Stelzer, E. H. K. (2008). Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Science, 322(5904), 1065–1069. https://doi.org/10.1126/science.1162493


Keller, P. J., & Stelzer, E. H. K. (2008). Quantitative in vivo imaging of entire embryos with Digital Scanned Laser Light Sheet Fluorescence Microscopy. Curr. Opin. Neurobiol., 18(6), 624–632. https://doi.org/10.1016/j.conb.2009.03.008


Klohs, J., Gräfe, M., Graf, K., Steinbrink, J., Dietrich, T., Stibenz, D., … Wunder, A. (2008). In vivo imaging of the inflammatory receptor CD40 after cerebral ischemia using a fluorescent antibody. Stroke, 39(10), 2845–2852. https://doi.org/10.1161/STROKEAHA.107.509844


Konopka, C. A., & Bednarek, S. Y. (2008). Variable-angle epifluorescence microscopy: a new way to look at protein dynamics in the plant cell cortex. Plant J., 53(1), 186–196. https://doi.org/10.1111/j.1365-313X.2007.03306.x


Laksameethanasan, D., Brandt, S. S., Engelhardt, P., Renaud, O., & Shorte, S. L. (2008). A Bayesian reconstruction method for micro-rotation imaging in light microscopy. Microsc. Res. Tech., 71(2), 158–167. https://doi.org/10.1002/jemt.20550


Munson, C., Huisken, J., Bit-Avragim, N., Kuo, T., Dong, P. D., Ober, E. A., … Stainier, D. Y. R. (2008). Regulation of neurocoel morphogenesis by Pard6 gamma b. Dev. Biol., 324(1), 41–54. https://doi.org/10.1016/j.ydbio.2008.08.033


Preibisch, S., Ejsmont, R., Rohlfing, T., & Tomancak, R. (2008). Towards digital representation of Drosophila embryogenesis. In Biomedical Imaging: From Nano to Macro, 2008. ISBI 2008. 5th IEEE International Symposium on (pp. 324–327). https://doi.org/10.1109/ISBI.2008.4540998


Preibisch, S., Rohlfing, T., Hasak, M. P., & Tomancak, P. (2008). Mosaicing of single plane illumination microscopy images using groupwise registration and fast content-based image fusion. In Medical Imaging (Vol. 6914, p. 69140E–69140E–8). International Society for Optics and Photonics. https://doi.org/10.1117/12.770893


Renaud, O., Viña, J., Yu, Y., Machu, C., Trouvé, A., Van der Voort, H., … Shorte, S. L. (2008). High-resolution 3-D imaging of living cells in suspension using confocal axial tomography. Biotechnol. J., 3(1), 53–62. https://doi.org/10.1002/biot.200700188


Reynaud, E. G., Krzic, U., Greger, K., & Stelzer, E. H. K. (2008). Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage. HFSP J., 2(5), 266–275. https://doi.org/10.2976/1.2974980


Ritter, J. G., Veith, R., Siebrasse, J.-P., & Kubitscheck, U. (2008). High-contrast single-particle tracking by selective focal plane illumination microscopy. Opt. Express, 16(10), 7142–7152.


Samsonov, D., Elsaesser, A., Edwards, A., Thomas, H. M., & Morfill, G. E. (2008). High speed laser tomography system. Rev. Sci. Instrum., 79(3), 35102. https://doi.org/10.1063/1.2885683


Scherz, P. J., Huisken, J., Sahai-Hernandez, P., & Stainier, D. Y. R. (2008). High-speed imaging of developing heart valves reveals interplay of morphogenesis and function. Development, 135(6), 1179–1187. https://doi.org/10.1242/dev.010694


Tokunaga, M., Imamoto, N., & Sakata-Sogawa, K. (2008). Highly inclined thin illumination enables clear single-molecule imaging in cells. Nat. Methods, 5(2), 159–161. https://doi.org/10.1038/nmeth1171


Turaga, D., & Holy, T. E. (2008). Miniaturization and defocus correction for objective-coupled planar illumination microscopy. Opt. Lett., 33(20), 2302–2304.


van ’t Hoff, M., de Sars, V., & Oheim, M. (2008). A programmable light engine for quantitative single molecule TIRF and HILO imaging. Opt. Express, 16(22), 18495–18504.