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LSFM (Light Sheet Fluorescent Microscopy)

This is a list of publications on the theory, design and applications of light sheet microscopy and similar techniques. We try to keep it up-to-date, but please email Susi (spower@morgridge.org) if a paper is missing.

2012 SPIM Publications

Baumgart, E., & Kubitscheck, U. (2012). Scanned light sheet microscopy with confocal slit detection. Opt. Express, 20(19), 21805–21814. https://doi.org/10.1364/OE.20.021805


Bhattacharya, D., Singh, V. R., Zhi, C., So, P. T. C., Matsudaira, P., & Barbastathis, G. (2012). Three dimensional HiLo-based structured illumination for a digital scanned laser sheet microscopy (DSLM) in thick tissue imaging. Opt. Express, 20(25), 27337–27347. https://doi.org/10.1364/OE.20.027337


Bourgenot, C., Saunter, C. D., Taylor, J. M., Girkin, J. M., & Love, G. D. (2012). 3D adaptive optics in a light sheet microscope. Opt. Express, 20(12), 13252–13261. https://doi.org/10.1364/OE.20.013252


Brede, C., Friedrich, M., Jordán-Garrote, A.-L., Riedel, S. S., Bäuerlein, C. A., Heinze, K. G., … Beilhack, A. (2012). Mapping immune processes in intact tissues at cellular resolution. J. Clin. Invest., 122(12), 4439–4446. https://doi.org/10.1172/JCI65100


Bruns, T., Schickinger, S., Wittig, R., & Schneckenburger, H. (2012). Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy. J. Biomed. Opt., 17(10), 101518. https://doi.org/10.1117/1.JBO.17.10.101518


Buytaert, J. A. N., Descamps, E., Adriaens, D., & Dirckx, J. J. J. (2012). The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens. Anat. Res. Int., 2012, 206238. https://doi.org/10.1155/2012/206238


Cutrale, F., & Gratton, E. (2012). Inclined selective plane illumination microscopy adaptor for conventional microscopes. Microsc. Res. Tech., 75(11), 1461–1466. https://doi.org/10.1002/jemt.22089


Ertürk, A., Becker, K., Jährling, N., Mauch, C. P., Hojer, C. D., Egen, J. G., … Dodt, H.-U. (2012). Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nat. Protoc., 7(11), 1983–1995. https://doi.org/10.1038/nprot.2012.119


Fahrbach, F. O., & Rohrbach, A. (2012). Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media. Nat. Commun., 3, 632. https://doi.org/10.1038/ncomms1646


Gao, L., Shao, L., Higgins, C. D., Poulton, J. S., Peifer, M., Davidson, M. W., … Betzig, E. (2012). Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens. Cell, 151(6), 1370–1385. https://doi.org/10.1016/j.cell.2012.10.008


Höckendorf, B., Thumberger, T., & Wittbrodt, J. (2012). Quantitative analysis of embryogenesis: a perspective for light sheet microscopy. Dev. Cell, 23(6), 1111–1120. https://doi.org/10.1016/j.devcel.2012.10.008


Huisken, J. (2012). Slicing embryos gently with laser light sheets. Bioessays, 34(5), 406–411. https://doi.org/10.1002/bies.201100120


Jorand, R., Le Corre, G., Andilla, J., Maandhui, A., Frongia, C., Lobjois, V., … Lorenzo, C. (2012). Deep and clear optical imaging of thick inhomogeneous samples. PLoS One, 7(4), e35795. https://doi.org/10.1371/journal.pone.0035795


Kaufmann, A., Mickoleit, M., Weber, M., & Huisken, J. (2012). Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope. Development, 139(17), 3242–3247. https://doi.org/10.1242/dev.082586


Keller, P. J., & Dodt, H.-U. (2012). Light sheet microscopy of living or cleared specimens. Curr. Opin. Neurobiol., 22(1), 138–143. https://doi.org/10.1016/j.conb.2011.08.003


Kirchmaier, B. C., Poon, K. L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., … Brand, T. (2012). The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development. Dev. Biol., 363(2), 438–450. https://doi.org/10.1016/j.ydbio.2012.01.015


Krzic, U., Gunther, S., Saunders, T. E., Streichan, S. J., & Hufnagel, L. (2012). Multiview light-sheet microscope for rapid in toto imaging. Nat. Methods, 9(7), 730–733. https://doi.org/10.1038/nmeth.2064


Mappes, T., Jahr, N., Csáki, A., Vogler, N., Popp, J., & Fritzsche, W. (2012). Die Erfindung des Immersions-Ultramikroskops 1912 – Beginn der Nanotechnologie? Angew. Chem. Int. Ed Engl., 124(45), 11370–11375. https://doi.org/10.1002/ange.201204688


Mellman, K., Huisken, J., Dinsmore, C., Hoppe, C., & Stainier, D. Y. (2012). Fibrillin-2b regulates endocardial morphogenesis in zebrafish. Dev. Biol., 372(1), 111–119. https://doi.org/10.1016/j.ydbio.2012.07.015


Niedworok, C. J., Schwarz, I., Ledderose, J., Giese, G., Conzelmann, K.-K., & Schwarz, M. K. (2012). Charting monosynaptic connectivity maps by two-color light-sheet fluorescence microscopy. Cell Rep., 2(5), 1375–1386. https://doi.org/10.1016/j.celrep.2012.10.008


Olarte, O. E., Licea-Rodriguez, J., Palero, J. A., Gualda, E. J., Artigas, D., Mayer, J., … Loza-Alvarez, P. (2012). Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles. Biomed. Opt. Express, 3(7), 1492–1505. https://doi.org/10.1364/BOE.3.001492


Opitz, R., Maquet, E., Huisken, J., Antonica, F., Trubiroha, A., Pottier, G., … Costagliola, S. (2012). Transgenic zebrafish illuminate the dynamics of thyroid morphogenesis and its relationship to cardiovascular development. Dev. Biol., 372(2), 203–216. https://doi.org/10.1016/j.ydbio.2012.09.011


Oshima, Y., Sato, H., Kajiura-Kobayashi, H., Kimura, T., Naruse, K., & Nonaka, S. (2012). Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope. Optical Society of America. Retrieved from https://www.osapublishing.org/abstract.cfm?URI=oe-20-15-16195


Rubio-Guivernau, J. L., Gurchenkov, V., Luengo-Oroz, M. A., Duloquin, L., Bourgine, P., Santos, A., … Ledesma-Carbayo, M. J. (2012). Wavelet-based image fusion in multi-view three-dimensional microscopy. Bioinformatics, 28(2), 238–245. https://doi.org/10.1093/bioinformatics/btr609


Schröter, T. J., Johnson, S. B., John, K., & Santi, P. A. (2012). Scanning thin-sheet laser imaging microscopy (sTSLIM) with structured illumination and HiLo background rejection. Biomed. Opt. Express, 3(1), 170–177. https://doi.org/10.1364/BOE.3.000170


Siebrasse, J. P., Kaminski, T., & Kubitscheck, U. (2012). Nuclear export of single native mRNA molecules observed by light sheet fluorescence microscopy. Proc. Natl. Acad. Sci. U. S. A., 109(24), 9426–9431. https://doi.org/10.1073/pnas.1201781109


Silvestri, L., Bria, A., Sacconi, L., Iannello, G., & Pavone, F. S. (2012). Confocal light sheet microscopy: micron-scale neuroanatomy of the entire mouse brain. Opt. Express, 20(18), 20582–20598. https://doi.org/10.1364/OE.20.020582


Spille, J.-H., Kaminski, T., Königshoven, H.-P., & Kubitscheck, U. (2012). Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue. Opt. Express, 20(18), 19697–19707. https://doi.org/10.1364/OE.20.019697


Takao, D., Taniguchi, A., Takeda, T., Sonobe, S., & Nonaka, S. (2012). High-speed imaging of amoeboid movements using light-sheet microscopy. PLoS One, 7(12), e50846. https://doi.org/10.1371/journal.pone.0050846


Taormina, M. J., Jemielita, M., Stephens, W. Z., Burns, A. R., Troll, J. V., Parthasarathy, R., & Guillemin, K. (2012). Investigating bacterial-animal symbioses with light sheet microscopy. Biol. Bull., 223(1), 7–20.


Taylor, J. M., Saunter, C. D., Love, G. D., & Girkin, J. M. (2012). Prospective gating for 3D imaging of the beating zebrafish heart in embryonic development studies. In SPIE BiOS (pp. 822716–822716–7). International Society for Optics and Photonics. https://doi.org/10.1117/12.908164


Temerinac-Ott, M., Ronneberger, O., Ochs, P., Driever, W., Brox, T., & Burkhardt, H. (2012). Multiview deblurring for 3-D images from light-sheet-based fluorescence microscopy. IEEE Trans. Image Process., 21(4), 1863–1873. https://doi.org/10.1109/TIP.2011.2181528


Tomer, R., Khairy, K., Amat, F., & Keller, P. J. (2012). Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy. Nat. Methods, 9(7), 755–763. https://doi.org/10.1038/nmeth.2062


Weber, M., & Huisken, J. (2012). Omnidirectional microscopy. Nat. Methods, 9(7), 656–657. https://doi.org/10.1038/nmeth.2022