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LSFM (Light Sheet Fluorescent Microscopy)

This is a list of publications on the theory, design and applications of light sheet microscopy and similar techniques. We try to keep it up-to-date, but please email Susi (spower@morgridge.org) if a paper is missing.

2009 SPIM Publications

Abbott, A. (2009). Microscopic marvels: Seeing the system. Nature, 459(7247), 630–631. https://doi.org/10.1038/459630a


Buytaert, J. A. N., & Dirckx, J. J. J. (2009). Tomographic imaging of macroscopic biomedical objects in high resolution and three dimensions using orthogonal-plane fluorescence optical sectioning. Appl. Opt., 48(5), 941–948.


Ermolayev, V., Friedrich, M., Nozadze, R., Cathomen, T., Klein, M. A., Harms, G. S., & Flechsig, E. (2009). Ultramicroscopy reveals axonal transport impairments in cortical motor neurons at prion disease. Biophys. J., 96(8), 3390–3398. https://doi.org/10.1016/j.bpj.2009.01.032


Friedrich, M., Nozadze, R., Gan, Q., Zelman-Femiak, M., Ermolayev, V., Wagner, T. U., & Harms, G. S. (2009). Detection of single quantum dots in model organisms with sheet illumination microscopy. Biochem. Biophys. Res. Commun., 390(3), 722–727. https://doi.org/10.1016/j.bbrc.2009.10.036


Herbert, S. P., Huisken, J., Kim, T. N., Feldman, M. E., Houseman, B. T., Wang, R. A., … Stainier, D. Y. R. (2009). Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation. Science, 326(5950), 294–298. https://doi.org/10.1126/science.1178577


Herzog, W., Müller, K., Huisken, J., & Stainier, D. Y. R. (2009). Genetic evidence for a noncanonical function of seryl-tRNA synthetase in vascular development. Circ. Res., 104(11), 1260–1266. https://doi.org/10.1161/CIRCRESAHA.108.191718


Hofman, R., Segenhout, J. M., & Wit, H. P. (2009). Three-dimensional reconstruction of the guinea pig inner ear, comparison of OPFOS and light microscopy, applications of 3D reconstruction. J. Microsc., 233(2), 251–257. https://doi.org/10.1111/j.1365-2818.2009.03115.x


Huisken, J., & Stainier, D. Y. R. (2009). Selective plane illumination microscopy techniques in developmental biology. Development, 136(12), 1963–1975. https://doi.org/10.1242/dev.022426


Jährling, N., Becker, K., & Dodt, H.-U. (2009). 3D-reconstruction of blood vessels by ultramicroscopy. Organogenesis, 5(4), 227–230.


Karaköylü, E. M., Franks, P. J. S., Tanaka, Y., Roberts, P. L. D., & Jaffe, J. S. (2009). Copepod feeding quantified by planar laser imaging of gut fluorescence. Limnol. Oceanogr. Methods, 7(1), 33–41. https://doi.org/10.4319/lom.2009.7.33


Keller, P. J., Schmidt, A. D., Wittbrodt, J., & Stelzer, E. H. K. (2009). The zebrafish digital embryo: in toto reconstruction of zebrafish early embryonic development with digital scanned laser light sheet fluorescence microscopy. In European Conferences on Biomedical Optics (Vol. 7367, p. 73670G–73670G–10). International Society for Optics and Photonics. https://doi.org/10.1117/12.831496


Krzic, U. (2009). Multiple-view microscopy with light-sheet based fluorescence microscope. Retrieved from http://archiv.ub.uni-heidelberg.de/volltextserver/9668/


Lee, H. K., Uddin, M. S., Sankaran, S., Hariharan, S., & Ahmed, S. (2009). A field theoretical restoration method for images degraded by non-uniform light attenuation : an application for light microscopy. Opt. Express, 17(14), 11294–11308.


Leischner, U., Zieglgänsberger, W., & Dodt, H.-U. (2009). Resolution of ultramicroscopy and field of view analysis. PLoS One, 4(6), e5785. https://doi.org/10.1371/journal.pone.0005785


Martinez-Morales, J. R., Rembold, M., Greger, K., Simpson, J. C., Brown, K. E., Quiring, R., … Wittbrodt, J. (2009). ojoplano-mediated basal constriction is essential for optic cup morphogenesis. Development, 136(13), 2165–2175. https://doi.org/10.1242/dev.033563


Preibisch, S., Saalfeld, S., Rohlfing, T., & Tomancak, P. (2009). Bead-based mosaicing of single plane illumination microscopy images using geometric local descriptor matching. In SPIE Medical Imaging (Vol. 7259, p. 72592S–72592S–10). International Society for Optics and Photonics. https://doi.org/10.1117/12.812612


Razansky, D., Vinegoni, C., & Ntziachristos, V. (2009). Imaging of mesoscopic-scale organisms using selective-plane optoacoustic tomography. Phys. Med. Biol., 54(9), 2769–2777. https://doi.org/10.1088/0031-9155/54/9/012


Rohrbach, A. (2009). Artifacts resulting from imaging in scattering media: a theoretical prediction. Opt. Lett., 34(19), 3041–3043. https://doi.org/10.1364/OL.34.003041


Santi, P. A., Johnson, S. B., Hillenbrand, M., GrandPre, P. Z., Glass, T. J., & Leger, J. R. (2009). Thin-sheet laser imaging microscopy for optical sectioning of thick tissues. Biotechniques, 46(4), 287–294. https://doi.org/10.2144/000113087


Stelzer, E. H. K. (2009). S09-02 Light sheet based fluorescence microscopes (LSFM, SPIM, DSLM) reduce phototoxic effects by several orders of magnitude. Mech. Dev., 126, Supplement(0), S36. https://doi.org/10.1016/j.mod.2009.06.1034